Clockwise screw the puncturing end with rubber sleeve into the holder.
Prepare venipuncture site with an appropriate antiseptic. Remove needle shield. Perform venipuncture with arm downward.
Push tube into needle, puncturing stopper diaphragm. Center tube in holder when penetrating the stopper.
When first tube has filled to its stated volume and blood flow ceases, remove it from holder. Place successive tubes in holder, puncturing diaphragm to begin flow.
